EMSTSFIG 1: Essential steps in embedding and trimming small pieces.

EMSTSFIG 1: Essential steps in embedding and trimming small pieces.

A. Embed fixed worm pieces in agar to position them close together (with uniform orientation).
B.
Embed the agar block into plastic resin in a mold and heat cure.
C.
Trim the block into a pyramidal shape by hand or using a glass knife on the microtome stage to make a close approach to the embedded worms.
D.
Final shape of the top of the block face just prior to thin sectioning. Long edge will be bottom of block during microtomy.

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