FITC Staining in C. elegans

FITC Staining of Amphid (ADL, ASH, ASI, ASJ, ASK, AWB) and Phasmid (PHA and PHB) Neurons by Hedgecock et al., 1985

1. A stock dye solution containing 20 mg/ml 5-fluorescein isothiocyanate in dimethylformamide can be stored at -20°C indefinitely.
2. 50 µl of this stock solution is then mixed with 200 µl of M9 buffer and applied evenly to the surface of a 10 ml NGM plate preseeded with a lawn of E. coli.
3. After 2 hr to allow the dye to diffuse into the agar, (final concentration 0.1 mg/ml), live animals are transferred to the plate.
4. After staining for 2 hr to overnight, the animals are transferred to a plate devoid of the dye for at least 10 min to remove free FITC from the intestine before viewing under the microscope.

See also Cell Biology and Methods in Cell Biology Chapters in Wormbook