Epithelial System of the Male (Part II) - Sensilla: The Post-Cloacal Sensilla

Post-cloacal sensilla composition and structure -Post-cloacal sensilla neuron connectivity - Back to Contents

Post-cloacal sensilla composition and structure

Immediately posterior of the cloaca are a left/right (L/R) bilateral pair of sensilla called the post-cloacal sensilla (PCS) (MaleEpiFIG22A-C). Like the hook these sensilla are embedded in sclerotized cuticle. Each post-cloacal sensillum contains the endings of 3 sensory-motor neurons, PCAL/R, PCBL/R and PCCL/R. In each sensillum the neurons are surrounded by sheath cells (PCshL/R) and socket cells (PCsoL/R). Sensilla also contains a third epithelial cell PCh (formed by fusion of PCh(L/R); MaleEpiTABLE4). Cells of the PCS are derived from the L1 rectal blast cells Y and B (MaleEpiFIG23).

The cell bodies of each left and right sensillum are grouped together in the left or right cloacal ganglia (CGL/R), located on either side of the proctodeum. Cell body positions are relatively invariant and can be most clearly observed in late L4 (MaleEpiFIG22B; see PCS Cell Identification Guide by L. R. Garcia).

MaleEpiTABLE4: Summary of PCS Cells

Cell type

Cell name Lineage name Syncytial No. nuclei Features
Sheath PCshL Y.plppv no 1  
  PCshR Y.prppv no 1  
Socket PCsoL Y.plap no 1  
  PCsoR Y.prap no 1  
Epithelia (other) PChL Y.plaa yes 2 (PChL and PChR)  
  PChR Y.praa yes    
Sensory-motor neuron PCAL Y.plppd no 1

short striated rootlet, basal body; dense ending under cuticle

  PCAR Y.prppd no 1 short striated rootlet, transition zone; dense ending under cuticle
  PCBL Y.plpa no 1 ends in sheath
  PCBR Y.prpa no 1 ends in sheath
  PCCL B.arpaaa no 1 long striated rootlet; crosses to innervate contra-lateral sensillum, ends in sheath
  PCCR B.alpaaa no 1 long striated rootlet; crosses to innervate contra-lateral sensillum, ends in sheath

 

From the CGL/R, PCS neurons send axons anteriorly into the preanal ganglion (PAG) (MaleEpiFIG25A) and dendrites posteriorly to the sensilla. PCBL/R neurons end in the sheath. PCAL/R cilia extend the furthest, ending in the cuticle. PCCL and PCCR neurons cross just posterior to the proctodeum and end in contra-lateral sensillum (MaleEpiFIG24A-C).

 

Post-cloacal sensilla neuron connectivity

Targets of the PCA, PCB and PCB neurons include each other, the hook neurons, spicule neurons SPCL/R and SPD, male-specific muscles and the gonad (The Male Wiring Project). These connections ensure that vulval location (by the PCS and hook) are coordinated with the subsequent steps of spicule insertion and sperm transfer (reviewed in WormBook: Male Mating Behavior - Barr and Garcia). PCS neuron dendrites form NMJs with male-specific oblique and gubernacular muscles en route to the sensilla (MaleEpiFIG25B; see also MALE HANDBOOK- MALE MUSCLE SYSTEM - PART II). Innervation of the oblique muscles may ensure that the tail adopts the appropriate posture for spicule prodding and insertion. Gubernacular muscle stimulation may facilitate spicule movement through the proctodeum by lifting the gubernaculum. In the preanal ganglion, PCBL/R and PCCL/R axons (and SPCL/R ) run on the dorsal surface of the ganglion and have synapses directed towards the gonad (MaleEpiFIG25C).


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