InterFIG 3: Arcade cell bodies are located around the procorpus and anterior bulb of the pharynx.
A. Epifluorescent image of the head of a transgenic animal expressing the F25B12.1::GFP reporter in arcade cells, medial left lateral view. Original magnification, 600x. (Strain source: The Genome BC C. elegans gene expression consortium; McKay et al., 2004.) Indicated are posterior (p) and anterior (a) arcade cell bodies. One of the anterior arcade cells is not visible in this focal plane. (Gray arrow) Process of a posterior arcade cell. |
B. DIC image of the same animal. Indicated are the nuclei of posterior (black arrowheads) and anterior (black arrows) arcade cells that are visible in this focal plane. (White arrows) Body wall muscle nuclei; (\white arrowheads) nuclei of anterior hypodermal (hyp 4, hyp 6/7) cells, which are larger than arcade nuclei; (bc) buccal cavity.
C. Overlay of DIC and epifluorescence images in A and B.
D. TEM, longitudinal section close to midline of the anterior of the head of an adult animal. Indicated are three posterior arcade cells located on the dorsal side and one posterior arcade cell located on the ventral side of the pharynx procorpus. Posterior arcade ring is becoming visible in front of the procorpus. (BWmu) Body wall muscle. At this plane, the myofilament lattice of one muscle cell reaches the tip of the nose. Bar, Bar, 1 μm. (Image source: [Hall] B156-7778.)
E. Epifluorescent image of an adult animal from a strain expressing the ajm-1::GFP reporter, lateral view. This marker gene is expressed at the apical borders of all epithelia. The two arcade rings (a) attach to each other and to neighboring tissues (pharyngeal epithelium [e] at the posterior, and hyp 1 at the anterior) via adherens junctions. Original magnification, 600x. (Strain source: H. Yu and P. W. Sternberg.)
F. DIC image of the same animal as in E, showing the anterior of the pharynx and buccal cavity. Distinct domains of the buccal cavity and the anterior hypodermis are covered by various pharyngeal and epithelial cells. Abbreviations are the same as in InterFIG 1A; (arrow) cuticular flaps.
G. Overlay of DIC and epifluorescence images in E and F.
Click on picture for full resolution image.