HypFIG 12 Tail hypodermis

HypFIG 12: Tail hypodermis.

A. Tail hypodermal cells, lateral view. (Red) hyp 8; (purple) hyp 9; (light green) hyp 10; (yellow) hyp 11. (Green circle) Phasmid opening; (s) seam. hyp 8-10 fit into one another in a succeeding fashion, whereas hyp 11 wraps over the dorsal side. hyp 8 and hyp 11 abut the hyp 7 syncytium at their anterior border and make adherens junctions and gap junctions to it. (Dark circles) Nuclei (based on Nguyen et al., 1999). Asterisks in panels A, C, D and G indicate posteriormost hyp 7 nuclei.
B.
Epifluorescent image of an L1-stage animal from a strain expressing the ajm-1::GFP reporter showing the apical borders of tail hypodermal cells, ventral view. Original magnification 600x. (Strain source: H. Yu and P. W. Sternberg.
C.
DIC image of the same animal as in B. Shown is the position of each hypodermal nucleus as well as the posteriormost seam cell.
D.
Epifluorescent image of an adult animal showing hyp 7 in tail.
E.
Epifluorescent image of an L2-stage animal from a strain expressing the ajm-1::GFP reporter showing the apical borders of tail hypodermal cells, left lateral view. Original magnification 600x. (Strain source: H. Yu and P. W. Sternberg.)
F. DIC image of the same animal as in D. The position of each hypodermal nucleus, as well as the posteriormost seam cell, is shown on the middle-left plane. The hyp 11 nucleus is becoming visible.
G.
DIC image of the same animal as in D and E (the middle plane). hyp 8-10 nuclei are visible, but the hyp 11 nucleus is not. The two post-anal, ventral hyp7 nuclei are seen, as well as nuclei of some lumbar ganglia neurons.

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